Journal: Pharmaceutics
Article Title: Evaluation of Adjuvant Activity and Bio-Distribution of Archaeosomes Prepared Using Microfluidic Technology
doi: 10.3390/pharmaceutics14112291
Figure Lengend Snippet: OVA- specific T cells as determined by ELISpot. Splenocytes of mice immunized with OVA antigen alone or formulated with SLA archaeosomes were collected on Day 28 (7 days post 2nd immunization). Cells were stimulated in vitro with OVA peptide SIINFEKL and IFN-γ secretion measured by ELISpot assay. Grouped data is presented as geometric mean ± SD (n = 4–5 per group). **** p < 0.0001.
Article Snippet: A peptide corresponding to CD 8+ T cell epitope OVA 257-264 : SIINFEKL (JPT Peptide Technologies GmbH, Berlin, Germany) was used as a stimulant.
Techniques: Enzyme-linked Immunospot, In Vitro